Contact an Account ManagerDetermination of plasma aminothiols by high performance liquid chromatography after precolumn derivatization with N-(2-acridonyl)maleimide
Summary
Type: HPLC
Application #: 122810
Detection:
Conditions: Eluent A: 20 mmol/L ammonium acetate + 1 mmol/L dibutylamine in water, pH 2.4 (adjusted with 35 % perchloric acid) – methanol (95:5, v/v)
Eluent B: 20 mmol/L ammonium acetate + 1 mmol/L dibutylamine in water, pH 2.4 (adjusted with 35 % perchloric acid) – acetonitrile (5:95, v/v)
Gradient: 20 % B for 6 min, 20–70 % B in 1 min, 70 % B for 3 min, 70–20 % B and 20 % B for 4 min
Flow rate: 1.5 mL/min
Injection volume: 20 µL
Authors: Benkova, B. et al.
Source: J. Chromatogr. B 870 (2008), 103–108
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